![]() ![]() Therefore, it is very difficult to measure slurry concentration by gravity with salt-based solutions. When you use these solutions, remember to rinse tubing and nozzles directly after packing to prevent clogging of the flow path.Īdding salt to packing solutions slows the settling of the resin beads and allows them to settle less tightly. Properties of Capto™ ImpRes resins in various packing solutionsĬapto™ ImpRes resins settle quickly in water, in 20% ethanol, and in salt solutions for packing. Because Chromaflow™ columns are pack-in-place columns, they have no registered consolidated bed heights, and the CF is used throughout the packing process. CF is used in the chromatography resin preparation step to calculate the resin volume needed to pack a desired bed height. When packing BPG and AxiChrom™ columns, PF is used in the packing procedure to calculate the final packed bed height after the consolidation step. L cons = consolidated bed height, that is, bed height measured after settling the resin at a given flow velocity L settled = bed height measured after settling by gravity For example, in water, when consolidated at 30 to 60 cm/h, the PF is 1.15 and the CF is 1.12 for Capto™ ImpRes.Įquations to calculate CF, PF, and column volume (CV) are shown below:Ĭompression factor, CF = L settled / L packed Therefore, you should separate the compression factor ( CF) from the packing factor ( PF). The height of a gravity-settled bed differs from the height of a bed settled under low flow (consolidated) due to the properties and the behavior of the resin under different conditions. General packing considerations Definitions The pressure-flow specification for Capto™ ImpRes in large columns is 220 cm/h at less than 3 bar (0.3 MPa, 43.5 psi) and at a 20 cm bed height (tested with water at 20☌ in a 1 m diameter column). The small average bead size of 40 µm gives you high resolution in your bioprocessing.Ĭapto™ ImpRes resins offer you a wide range of bed heights and flow velocities. Our Capto™ ImpRes resins are based on highly rigid agarose that allows high flow velocities, which is essential for raising productivity in large-scale operations. Capto™ ImpRes chromatography resin characteristics ![]() Result examples in the three packed columns, respectively are seen in the accordions at the bottom of this document. Best practices for packing MabSelect PrismA™ can be found in this article. This article explains best practices to help you efficiently pack Capto™ SP ImpRes, Capto™ Q ImpRes, Capto™ MMC ImpRes, and Capto™ adhere ImpRes chromatography resins in our large-scale AxiChrom™, BPG, and Chromaflow™ chromatography columns. Robust and verified packing and testing methods can eliminate such concerns and risks. ![]() Poorly packed columns can lead to costly disruptions and loss of your valuable product. To achieve effective purification, you need to be sure that the resin is efficiently packed. You can pack Capto™ ImpRes in large-scale columns to allow high flow velocities and high bed heights and increase the productivity and flexibility in your process design. These resins are based on high-flow agarose and offer excellent resolution in bioprocessing. ReadyToProcess Capto adhere ImpRes columns, 1 L and larger columns for clinical manufacturing that minimize the need for cleaning and cleaning validation.Capto™ ImpRes ion exchange chromatography resins are designed for high-throughput intermediate purification and polishing steps of a wide range of biomolecules in bioprocess applications. Process Characterization Kit Capto adhere ImpRes, designed for studying the potential impact that resin ligand density might have on the process outcome. HiTrap Capto adhere ImpRes columns, for easy use with a syringe, peristaltic pump, or chromatography system. Lab scale: prepacked for automated purification and analysis Lab scale: prepacked for manual purification
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